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DEPARTMENT OF MICROBIOLOGY AND RHIZOSPHERE

Kierownik

prof. dr hab. Lidia Sas-Paszt
tel.: +48 46 834 52 35, kom. 503 158 903
e-mail: Lidia.Sas@inhort.pl


SCOPE OF RESEARCH CONDUCTED

  • Development of innovative methods for soil and plant microbiome analysis, root research and bio-physico-chemical processes occurring in the plant rhizosphere (pH changes in the rhizosphere, root exudates, manganese reduction, iron reduction, aluminum ion detoxification).
  • Isolation, biochemical characterization and molecular identification of beneficial microorganisms from the rhizosphere of cultivated and wild plants as components of microbiological biopreparations.
  • Developing effective and safe methods for storing beneficial microorganisms in SYMBIOBANK for their further use.
  • Identification of soil and endophytic microorganisms using the assessment of phenotypic features and molecular methods: obtaining DNA profiles using the rep-PCR technique, DNA sequence analysis of selected genes, PCR-DGGE technique, directly in the soil environment and in roots, without the need to isolate microorganisms and multiply them on microbiological media.
  • Development of innovative microbiological consortia with biostimulating and protective effects in horticultural and agricultural crops and increasing plant adaptation to drought stress conditions.
  • Development of microbiological biopreparations accelerating the composting of brown coal and agricultural waste through biodegradation of organic matter.
  • Development of techniques for enriching organic fertilizers, mineral fertilizers and organic waste products with microorganisms.
  • Development of microbiological bioproducts: biofertilizers, biostimulants, soil improvers, organic mulches, growing media, microbiologically enriched composts for horticulture and agriculture.
  • Optimization of methods of using microbiological preparations in ecological and integrated production, for crop species (doses, dates).
  • Development of methods for verifying the effectiveness of the influence of consortia of beneficial microorganisms and microbiological biopreparations on the growth, yield of crop plants and their resistance to unfavorable biotic and abiotic factors, in laboratory, greenhouse, container and field experiments.
  • Assessment of the state of soil degradation in areas of intensive cultivation of horticultural and agricultural plants, based on microbiological and biochemical tests.
  • Analyses of the numbers of selected groups of microorganisms in soils, growing media, microbiological biopreparations, biofertilizers, biostimulants, in media from aquaponic crops and in water. 
  • Study of the activity and functional diversity of microorganisms in samples of microbiological biopreparations, soils and growing media.
  • Study of soil-plant-microorganism interactions based on:
    • assessment of the growth dynamics and morphology of shoots and roots of horticultural plants, using destructive (root scanner) and non-destructive techniques in situ (minirhizotron technique).
    • obtaining and analyzing root secretions and soil reaction in the plant rhizosphere.
    • assessment of the degree of colonization of roots of horticultural and agricultural plants by microorganisms, including the study of the degree of colonization of roots by mycorrhizal fungi, their morphotypes in the roots of horticultural, agricultural and forest plants.
    • determining the number of formed spores of mycorrhizal fungi in the rhizosphere of plants.
    • determining the degree of colonization of the rhizosphere and phyllosphere by selected groups of beneficial and pathogenic microorganisms.
    • assessment of photosynthetic efficiency in plant leaves based on chlorophyll fluorescence measurements.
    • studies of soil microorganism respiration using the Automatic CO2 Exchange Monitoring System2.
    • evaluation of vegetative growth parameters and plant biomass and yield.
  • Research on the use of rhizosphere bacteria and filamentous fungi, including Trichoderma in horticultural crops.
  • Development of technology for multiplying microorganisms on a semi-technical and industrial scale, in bioreactors in liquid media and on solid organic carriers.
  • Assessment of microbiological contamination in plant products and development of methods for extending shelf life and improving the sanitary condition of these products.
  • Use of plant compounds to combat fungal and bacterial pathogens.
  • Optimization of methods for breaking seed dormancy in horticultural plants.
  • Improving seed quality using beneficial microorganisms, phytohormones and secondary metabolites. 

Researchers

  • Prof. Dr. Hab. Lidia Sas-Paszt
  • Dr. hab. Magdalena Szczech, prof. IO
  • Dr. Eng. Edyta Derkowska
  • Dr. Sławomir Gluszek
  • Dr. hab. Beata Kowalska
  • Dr. Eng. Michał Oskiera
  • Dr. Beata Sumorok
  • Dr. Pawel Trzcinski

Technical staff

  • MSc Eng Mateusz Frąc
  • MSc Eng Anna Pałeczka
  • mgr michał przybyl
  • MSc Eng Krzysztof Weszczak
  • Catherine Kroc
  • Anna Michaelska
  • Ilona Skiba
  • Jolanta Winciorek
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