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Nursery methodologies for the regulation of the Ministry of Agriculture and Rural Development

Nursery methods to the Regulation of the Minister of Agriculture and Rural Development of 31 March 2017 on detailed requirements for the production and quality of nursery material

1. Overview card of nursery methods for apple, pear and quince (31.12.2025)
2. Overview card of nursery methods for peach, sweet cherry, plum and sour cherry (31.12.2025)
3. Overview card of nursery methods for strawberries and wild strawberries (31.12.2024)
4. Overview card of nursery methods for gooseberries, blackcurrants and redcurrants (31.12.2023)
5. Overview card of nursery methods for raspberry, blackberry and other Rubus ssp. species (31.12.2025)
6. Overview card of nursery methods for hazel and walnut (31.12.2022)
7. Overview card of nursery methods for highbush blueberries and cranberries (31.12.2025)
  1. Methodology of conducting observations for the presence of the cotton aphid (Eriosoma lanigerum) (Hemiptera:Aphididae) in mother plantations (scarlet orchards, seed orchards, rootstock nurseries) and in apple nurseries (Malus spp.) – Ms.28.01.Baw_kor_jab
  2. Methodology of conducting observations for the presence of the cotton aphid (Eriosoma lanigerum) (Hemiptera: Aphididae) in mother plantations (scarlet orchards, seed orchards, rootstock nurseries) and in pear and quince nurseries (Pyrus spp. and Cydonia oblonga) – Ms.29.01.Baw_kor_gr_pig
  3. Methodology of sampling nursery material for the presence of nematodes (Aphelenchoides spp.) on strawberries and wild strawberries – Ms.30.01.Wegorki
  4. Methodology of sampling from mother plantations for the presence of blackcurrant bud mite – (Cecidophyopsis ribis) – Ms.31.01.wp
  5. Methodology of sampling nursery material for laboratory tests for the presence of phytoplasmas on apple trees – Ms.32.01.fj
  6. Methodology for sampling nursery material for laboratory tests for the presence of phytoplasmas on pear and quince – Ms.33.01.fgp
  7. Methodology of sampling nursery material for laboratory tests for the presence of phytoplasmas on apricot – Ms.34.01.fm
  8. Methodology for sampling nursery material for laboratory tests for the presence of phytoplasmas on bird cherry and sour cherry – Ms.35.02.fca
  9. Methodology of sampling nursery material for laboratory tests for the presence of phytoplasmas on domestic plum and Japanese plum – Ms.36.01.fsdsj
  10. Methodology of sampling nursery material for laboratory tests for the presence of phytoplasmas on peach – Ms.37.01.fb
  11. Methodology of sampling nursery material for laboratory tests for the presence of Erwinia amylovora bacteria on apple trees – Ms.21.02.Erw_jab
  12. Methodology of sampling nursery material for laboratory testing for the presence of Erwinia amylovora bacteria on pear and quince – Ms.27.01.Erw_gp
  13. Methodology of sampling nursery material for laboratory testing for the presence of Pseudomonas syringae pv. morsprunorum on morel_A2023 – Ms.26.02.Psm
  14. Methodology for sampling nursery material for laboratory tests for the presence of viruses (+PPV) – Sweet cherry and sour cherry and species used as their rootstocks – Ms.25.01.WCW
  15. Methodology of sampling nursery material for laboratory tests for the presence of viruses (+PPV) and viroids – Peach and species used as its rootstocks – Ms.22.01.WWdB
  16. Methodology of sampling nursery material for laboratory tests for the presence of viruses (+PPV) – Apricot and species used as its rootstocks – Ms.23.01.WM
  17. Methodology of sampling nursery material for laboratory tests for the presence of viruses – House plum, Japanese plum and other Prunus L. species/hybrids susceptible to plum pox virus_A2023 – Ms.24.02.WS
  18. Methodology for sampling nursery material for laboratory testing for the presence of fungus Colletotrichum acutatum – Ms.20.01.CollF
  19. Methodology of sampling nursery material and water for laboratory testing for the presence of Phytophthora cactorum – Ms.19.01.PhF
  20. Methodology of sampling nursery material for testingtlaboratory tests for the presence of viruses – Sweet cherry, sour cherry and species used as their rootstocks – Ms.18.01.WCW
  21. Methodology of sampling nursery material for laboratory tests for the presence of viruses – Pear, Apple and Quince – Ms.01.01.WGJP
  22. Methodology of sampling nursery material for laboratory tests for the presence of viroids – Pear, Apple and Quince – Ms.02.01.WdGJP
  23. Methodology of collecting soil samples for the presence of longipetals (Longidorus spp.) and daggers (Xipinema spp.) – Ms.06.01.N
  24. Methodology of sampling for the presence of the Morus mulberry scale (Pseudaulacaspis pentagona) from the mother plantations Prunus, juglans i Currant. – Ms.07.01.T
  25. Methodology of sampling nursery material for laboratory tests for the presence of viruses – Truskawka_A2023– Ms.08.02.WT
  26. Methodology of sampling nursery material for laboratory tests for the presence of viruses – Blackcurrant, redcurrant and gooseberry – Ms.09.01.WPA
  27. Methodology of sampling nursery material for laboratory tests for the presence of viruses – Highbush blueberry and cranberry – Ms.10.01.WBŻ
  28. Methodology of sampling nursery material for laboratory tests for the presence of viruses – Raspberry, Blackberry – and other Rubus ssp. species._A2023 – MS.11.02.WMJ
  29. Methodology of sampling nursery material for laboratory tests for the presence of viruses – Leszczyna – Ms.12.01.WL
  30. Methodology for sampling nursery material for laboratory tests for the presence of viruses – Walnut – Ms.13.01.WOw
  31. Methodology of sampling nursery material for laboratory tests for the presence of phytoplasmas – Highbush blueberry and cranberry_A2023 – Ms.14.02.FBŻ
  32. Methodology of sampling nursery material for laboratory tests for the presence of phytoplasmas – Strawberry – Ms.15.01.FT
  33. Methodology for sampling nursery material for laboratory tests for the presence of phytoplasmas – Raspberry, Blackberry and other species Rubus_A2023– Ms.16.02.FR
  34. Methodology of sampling nursery material for laboratory tests for the presence of phytoplasmas – Leszczyna – Ms.17.01.FL
  35. Methodology of sampling nursery material for laboratory evaluation for the presence of Xanthomonas fragariae – Strawberry – MS.38.01.BT
  36. Methodology of sampling nursery material for the presence of the strawberry mite Phytonemus pallidus – Strawberry, Truskawka – Ms.39.01.RTr
  37. Methodology of sampling nursery material for the presence of the currant leaf midge (Dasineura tetensi) – Blackcurrant, Redcurrant, Gooseberry – Ms.40.01.PPLiś
  38. Methodology of sampling nursery material for the presence of raspberry stem midge (Resseliella theobaldi) – Malina, Jeżyna – Ms.41.01.PNŁod
  39. Methodology of sampling nursery material for laboratory evaluation for the presence of fungus Neofabraea alba –A2024_Ms.42.02.Nalba
  40. Methodology of sampling nursery material and water for laboratory tests for the presence of Phytophthora spp. – Thornless blackberry, True raspberry – Ms.43.01.PhyRub
  41. Methodology of sampling nursery material and water for laboratory tests for the presence of Phytophthora fragariae – Strawberry, Strawberry – Ms.44.01.PhyRub
  42. Methodology of sampling nursery material for laboratory testing for the presence of the fungus Diaporthe vaccinii – Highbush blueberry, Cranberry – Ms.45.01.Dvac
  43. Methodology for sampling nursery material for laboratory evaluation for the presence of the fungus Verticillium albo-atrum – A2024_Ms.46.02.Va-a
  44. Methodology for sampling nursery material and soil for laboratory evaluation of the presence of the fungus Verticillium dahliae – A2024_Ms.47.02.Vd
  45. Methodology of sampling nursery material for laboratory evaluation for the presence of Agrobacterium tumefaciens – Ms.48.01.At
  46. Methodology for sampling nursery material for laboratory evaluation for the presence of Exobasidium vaccinii – Ms.49.01.Ev
  47. Methodology of sampling nursery material for laboratory evaluation of the presence of the fungus Godronia cassandrae – Ms.50.01.Gc
  48. Methodology for sampling nursery material and water for laboratory evaluation of Phytophthora ramorum – Ms.51.01.Phr
  49. Methodology for sampling nursery material for laboratory evaluation for the presence of Verticillium dahliae – Ms.52.01.Vd
  50. Methodology for sampling nursery material for laboratory evaluation for the presence of Verticillium albo-atrum – Ms.53.01.Va-a_f
  51. Methodology for sampling nursery material for laboratory testing for the presence of Tobacco ring spot virus (TRSV) – highbush blueberry and cranberry. Ms.54.01.TRSV-Vaccinium.
  52. Methodology for sampling nursery material for laboratory testing for the presence of Tomato ringspot nepovirus (ToRSV) – apple. Ms.55.01.ToRSV-Malus.
  53. Methodology for sampling nursery material for laboratory testing for the presence of Tomato ringspot virus (ToRSV) - almond, plum, Japanese plum, sweet cherry, sour cherry, apricot, and other Prunus species. Ms.56.01.ToRSV-Prunus.
  54. Methodology for sampling nursery material for laboratory testing for the presence of Tomato ringspot nepovirus (ToRSV) – highbush blueberry and cranberry. Ms.57.ToRSV-Vaccinium.
  55. Ms.58.01. Methodology for sampling nursery material for laboratory testing for the presence of Tomato ringspot nepovirus (ToRSV) – raspberry, blackberry and other Rubus ssp. species. Ms.58.01.ToRSV-Rubus.
  56. Methodology for sampling nursery material for laboratory evaluation for the presence of the fungus Pucciniastrum minimum – highbush blueberry. Ms.59.01.P.minimum-Vaccinium.

RT-PCR reaction parameters for nursery methods

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